In vitro preservation of Cyrtopodium schargelliiusing the minimal growth technique

Introduction: Cyrtopodium schargellii, known in certain areas as ‘lluvia dorada’, is an orchid native to Central America, distributed in countries such as Venezuela and Colombia. It is possible to find this orchid in Colombia in the department of Córdoba and other surrounding areas. Objective: The objetive of this research was to establish a viable and effective protocol for the in vitro conservation of the orchid C. schargellii; Materials and methods: for this purpose, seedlings of this species previously germinated in vitro were used, and then explants were taken and sown in culture media supplemented with 100% MS salts and two osmoregulators (mannitol and sorbitol) in four treatments for each substance: T1 (0.5%), T2 (1%), T3 (1.5%) and T4 (2%). In addition, a control was prepared (T0, without osmoregulators and with 100% MS salts). Results: In the case of mannitol, shoot and root formation was observed, accompanied by chlorosis and death of some explants, while in the case of sorbitol, little shoot and root formation was observed, but all explants survive. Conclusion: In conclusion, the in vitro preservation protocol given by 0.5% sorbitol was the best treatment for the preservation of this orchid.

Keywords: Biotechnology, tropical dry forest, explants, growing media, orchid, osmoregulators.

https://doi.org/10.47499/revistaaccb.v1i37.331